Wonder if gas tank is sliding a bit? should be easy enough to tell with some prying.
check bed mount bolts and see if anything looks like its moving.
Are the rubber bumpers in the endgate?
How about running a chain to the hitch and hooking th other end to a garden tractor or? and giving it a tug sideways just enough to put side pressure on the rear end and lay under the back end to listen.
Need someone you trust pulling, and good on a clutch.
If you have rear opened, check end of axles do these use clips? I have not been in a newer one.